Do N-terminal nucleophile hydrolases indeed have a single amino acid catalytic center?
نویسندگان
چکیده
منابع مشابه
A Catalytic Mechanism for Cysteine N-Terminal Nucleophile Hydrolases, as Revealed by Free Energy Simulations
The N-terminal nucleophile (Ntn) hydrolases are a superfamily of enzymes specialized in the hydrolytic cleavage of amide bonds. Even though several members of this family are emerging as innovative drug targets for cancer, inflammation, and pain, the processes through which they catalyze amide hydrolysis remains poorly understood. In particular, the catalytic reactions of cysteine Ntn-hydrolase...
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Recent studies have shown that a number of glycoside hydrolase families do not follow the classical catalytic mechanisms, as they lack a typical catalytic base/ nucleophile. A variety of mechanisms are used to replace this function, including substrate-assisted catalysis, a network of several residues, and the use of non-carboxylate residues or exogenous nucleophiles. Removal of the catalytic b...
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Levvy, G. A. (1948). Biochem. J. 42, 2. Levvy, G. A. (1952). Biochem. J. 52, 464. Levvy, G. A. (1954). Biochem. J. 58, 462. Levvy, G. A. & Marsh, C. A. (1952). Biochem. J. 52, 690. Lohmar, R., Dimler, R. J., Moore, S. & Link, K. P. (1942). J. biol. Chem. 143, 551. Marsh, C. A. (1952). J. chem. Soc. p. 1578. Marsh, C. A. (1954). Biochem. J. 58, 609. Marsh, C. A., Alexander, F. & Levvy, G. A. (19...
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The HhaI methyltransferase recognizes the sequence GCGC and transfers a methyl group to C5 of the first cytosine residue. All m5C-methyltransferases contain a highly conserved sequence motif called the P-C motif. The cysteine residue of this motif is involved in catalysis by forming a covalent bond with the 6-position of cytosine prior to methyl group transfer. For the EcoRII methyltransferase,...
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ژورنال
عنوان ژورنال: FEBS Journal
سال: 2009
ISSN: 1742-464X,1742-4658
DOI: 10.1111/j.1742-4658.2009.06987.x